For bladder cancer cells 5637 and HT1376, we used flow cytometry analysis based on Annexin V staining, as shown in Figure 3D, 72 h post transfection, the average early apoptosis rates (Annexin V-PE+/7-AAD−) of FBLN1-transfected 5637 and HT1376 cells were 31.12% and 17.91% respectively, which were significantly higher when compared to the control vector-transfected cells (10.04% and 9.64%, P < 0.05). The gene discussed is ANXA5; the disease is urinary bladder carcinoma.