Ucn3 and insulin expression in β cells of T2D mice were examined by immunostaining on pancreata of obese diabetic (LepOb/Ob and LeprDb/Db) mice and from insulin-dependent diabetic mice (Ins2Akita), and compared to pancreata of age matched non-diabetic (C57BL/6) mice. This evidence concerns the gene INS and type 2 diabetes mellitus.