LAMP1 and infection: To address the question whether CCHFV localizes to these endosomal compartments during infection, we infected cells with CCHFV for 120 min and then stained them with antibodies detecting either (i) N and ALG-2-interacting protein X/apoptosis-linked-gene-2-interacting protein 1 (Alix/Aip1), which associates with MVBs to coordinate vesicle formation and biogenesis [31]; or (ii) N and Lamp1.