A total of eighteen mRNA, such as mgat5, st3gal1, st8sia4, were up-regulated in the HCC cell lines with different metastatic potentials (MHCC97L, MHCC97H and HCCLM3) compared with the non-metastatic HCC cell line Hep3B, while fifteen mRNA like c1galt1, gcent4, man1a1 were down-regulated (Table 2 and 3). Here, ST8SIA4 is linked to hepatocellular carcinoma.