Although a brief report of the anti-dementia or anti-ischemic activity of GV20 acupuncture stimulation has been published, whether GV20 acupuncture stimulation therapeutic efficacy in alleviating spatial cognitive function following repeated SCO-induced neuronal impairment is due to cholinergic system regulation or BDNF and CREB expression remains unknown [21]. The gene discussed is CREB1; the disease is dementia.