This also indicated that the expression of ChAT, BDNF and CREB-immunopositive neuronal cells in the hippocampus of rats treating acupuncture stimulation of GV20, but not of TE4 or the tail, restored memory impairment-related cholinergic function, and activated BDNF and CREB expression. This evidence concerns the gene CREB1 and memory impairment.