We found that synthetic Poly-G3 and natural TLR8 ligand (ssRNA40) completely reversed the suppressive functions of naturally occurring CD4+CD25+ Treg, and tumor-derived CD4+, CD8+, and γδ Treg cells (Kiniwa et al, 2007; Peng et al, 2005, 2007). This evidence concerns the gene CD4 and neoplasm.