We analyzed 263 patients pooled in 4 studies to assess whether the aberrant RUNX3 methylation/expression in serum/cancer tissues DNA was associated with advanced stage, including tumor size (T1–T2 vs T3–T4), lymph node involvement, lymph and blood vessels metastasis, and recurrence in esophageal carcinomas. This evidence concerns the gene RUNX3 and neoplasm.