These findings are consistent with previous studies that reported elevated anxiety-related behaviors in gonadally intact and OVX βERKO female mice in the elevated plus maze, OFT (Krezel et al., 2001; Imwalle et al., 2005) and light-dark transition tests (Tomihara et al., 2009), which suggests an ERβ involvement in anxiolysis in females. This evidence concerns the gene ESR2 and Anxiety.