To further investigate CTLA4-FasL mode-of-action in cancer cell line, we studied if CTLA4-FasL cytotoxic effect can be abrogated by the pan-caspase inhibitor (Z-VAD), caspase 8 inhibitor (Z-IETD-FMK) and caspase 9 inhibitor (Z-LEHD-FMK) on malignant cell lines positive for Fas only. This evidence concerns the gene CTLA4 and cancer.