To examine the influence of caspase-2 on poly(I:C) transfection-induced apoptosis and activation of other caspases, caspase-2 was knocked down selectively using caspase-2 siRNA (Figure 4c), which consequently decreased apoptosis (Figure 4d, Additional file 1: Figure S1), as well as caspase-3, -8, and -9 activation in poly(I:C)-transfected RCC cells (Figure 4e). This evidence concerns the gene CASP2 and renal cell carcinoma.