Thus, due to the challenge of examining the dynamics of multiple signaling pathways simultaneously in co-culture experiments alone, for the present study we integrated both experimental and computational approaches to elucidate the roles of VCAM-1, IL-8, and IL-1β in junction disassembly, actin remodeling, and actinomyosin contractility in melanoma-induced endothelial cell-cell junction disruption. The gene discussed is CXCL8; the disease is melanoma.