To determine the cut-off of the novel CytoBead ANCA for PR3- and MPO-ANCA, 465 human sera of patients and controls including 118 patients with ANCA-associated vasculitis, 133 with RA, 49 with ID, 20 with CD, 20 with AIH and 125 BD were run with the Aklides IIF interpretation system. Here, MPO is linked to Behcet disease.