Three non-synonymous variants in cardiac disease-associated genes were selected for co-segregation analysis in family members as they affected conserved residues in genes associated with primary cardiac pathologies: Ala119Thr in ACTN2, previously reported in a family with heterogeneous HCM [14], Ala572Asp in SCN5A (rs36210423), and Asp26Asn in plakophillin 2 (PKP2) (rs143004808). This evidence concerns the gene SCN5A and heart disorder.