Using the in vivo study of Tumbar et al. [16] as a prototype, we constructed a tetracycline-inducible plasmid system expressing fused Histone B2 with Green Fluorescent Protein (H2B-GFP) and generated stably transfected clonal HBL and SK-Mel28 human melanoma cell lines (HBL-H2B-GFP and SK-Mel28-H2B-GFP, respectively). Here, H2BC21 is linked to melanoma.