Collectively, all the above data demonstrated that melanoma cell lines contain a small pool of GFPhighABCB5highCD271high, quiescent/slow-cycling, self-renewing, melanoma stem-like cells that lose their ability to differentiate when targeted by TNF, even transiently, and that these cells appear to transfer this effect to further generations and manifest post-TNF exposure. Here, TNF is linked to melanoma.