A significant (p < 0.001) reduction in the sphere-forming capacity of melanosphere cells generated in the presence of LY204002 (Figure 5C) demonstrated that the sustained inactivation of AKT signaling reduced the melanoma SC compartment most likely by switching from symmetric to asymmetric self-renewal and by releasing the TNF-suppressed differentiation fate of melanoma SCs. This evidence concerns the gene AKT1 and melanoma.