Relative gene expression of α-klotho and renin-angiotensin system (RAS) compounds after 90 days evaluated in Control rats (C), Vitamin D deficient rats (VDD), rats submitted to Ischemia/Reperfusion Injury (IRI), and Vitamin D deficient rats submitted to Ischemia/Reperfusion Injury (VDD+IRI). The gene discussed is REN; the disease is injury.