Immunoblotting analyses showed that phosphorylated MEK and ERK compared to total ERK, as well as phosphorylated SMAD2/3 compared to total SMAD2/3 and SMAD4, were increased both in SNORD113-1 siRNA transfected HepG2 cells and in HCC tumor tissues. The gene discussed is SMAD4; the disease is hepatocellular carcinoma.