CUX1 and cancer: To verify whether CUX1 is required for expression of Wnt genes in these human cancer cells, we performed siRNA-mediated knockdown of CUX1 in six cell lines, two each from breast (MDA157 and MDA231), ovarian (PA-1 and SKOV3) and lung (H2347 and A427), and we performed RT-PCR analysis to measure the expression of Wnt genes previously identified as transcriptional targets of CUX1 in chromatin immunoprecipitation (ChIP) assays: Wnt 1, 2, 4, 6, 8b and 10a (Cadieux et al., 2009; Harada et al., 2008; and unpublished observations).