For example, CUX1 did not stimulate the expression of WNT5A in MCF10A cells, although this gene was upregulated in two MMTV-CUX1 mammary tumors and was previously reported to be activated by CUX1 in the PANC1 and HT1080 cell lines (Figs 4 and 6) (Ripka et al., 2007). This evidence concerns the gene WNT5A and breast cancer.