In this observation, the effects of trace residues of bovine CAPN1 were also confirmed by the fact that CAPN1/CAST4 complex stability has a greater impact on the enzyme origin of disease mutations (R385H, D600G related with the LGMD-2A) than the binding affinity with CAST4. Here, CAPN1 is linked to autosomal recessive limb-girdle muscular dystrophy type 2A.