Losartan treatment for 10 weeks in db/db mice can ameliorate key factors of DN, and there was no change in the H3K4me1 recruitment at PAI-1 and RAGE promoters, suggesting that it cannot reverse H3K4me changes observed in the db/db mice and accounting for the incomplete inhibitory effect of ARBs in DN patients [44]. The gene discussed is SERPINE1; the disease is liver dysplastic nodule.