A study result of ChIP assays from type 2 diabetes animal model db/dbH2O mice shows that H3K27me3 levels at RAGE and PAI-1 promoters were decreased compared with db/+H2O, and Losartan treatment had little or no effect on DN-related H3K27me3 levels at the above two genes promoters [44]. This evidence concerns the gene SERPINE1 and type 2 diabetes mellitus.