EFEMP1 and posterior cortical atrophy: To confirm MSP results and to obtain detailed information about the methylation status of CpG sites, bisulfite sequencing PCR (BSP) was performed in one normal immortalized prostatic cell line – PNT2 – and five PCa cell lines – 22Rv1, DU145, LNCaP, PC-3 and VCaP for EFEMP1, using primers that span the region of interest 17.