To uncover the biological role of fibulin-3, the protein encoded by EFEMP1, in PCa, PC-3 and LNCaP cells were transiently transfected with transfection-ready DNA containing EFEMP1 transcript variant 1 (Origene Technologies), following the manufacturer’s instructions, for 48 h (the time-point found to correspond to the highest EFEMP1 expression). The gene discussed is EFEMP1; the disease is posterior cortical atrophy.