Compared with control shRNA (Ctrl shRNA), the cells treated with Rpb3 shRNA1 and Rpb3 shRNA2 grew more slowly in vitro (as determined through MTT assay and BrdU assay) in both HCC-LM3 and SMMC-7721 cells (Fig. 2G and 2H). The gene discussed is POLR2C; the disease is hepatocellular carcinoma.