Using qRT-PCR assay, we found that overexpression of Rpb3 inhibited the mRNA level of E-cadherin in HepG2 cells (Fig. 3C), whereas knockdown of Rpb3 enhanced the mRNA level of E-cadherin in HCC-LM3 cells (Fig. 3D). The gene discussed is POLR2C; the disease is hepatocellular carcinoma.