To confirm that Rpb3 regulated E-cadherin transcription, we used E-cadherin promoter luciferase reporter assay, and found that overexpression of Rpb3 inhibited E-cadherin promoter transcription in HepG2 cells (Fig. 3E), whereas knockdown of Rpb3 enhanced E-cadherin promoter transcription in HCC-LM3 cells (Fig. 3F). This evidence concerns the gene CDH1 and hepatocellular carcinoma.