EGFR and large cell carcinoma: The reasons to use two most commonly used lung cancer cell lines A549 and H460 include the following: (i) they have different histologic subtypes, that is, A549 is adenocarcinoma and H460 is large cell carcinoma, although both belong to non-small-cell carcinoma; (ii) the origin of A549 cell was obtained from lung tissue and H460 was from lung pleural effusion, which may represent different stages of lung cancer; and (iii) both cells are EGFR wild type that could be tested by the drugs potentially effective for intrinsic EGFR-TKI resistance.