ANCOVA (between-subjects factor: PCOS, control) showed no main effects of PCOS F(1,92) = 0.115, p = 0.736), but revealed that it is the insulin concentration that mainly determines afamin value F(1.92) = 10.06, p = 0.002, ηp2 = 0.10. Here, AFM is linked to polycystic ovary syndrome.