EDA and tooth agenesis: This being located with in the monomer-monomer interface plays critical role during receptor binding activity of EDA and several other TNF domain containing protein.[34], [25], [35] Several studies showed that mutations which affect the structural stability of EDA protein mainly contribute in X linked hypohidrotic ectodermal dysplasia (XLHED) on the other hand mutations which slightly alter the receptor binding activity have their role in milder form of XLHED and/or non-syndromic tooth agenesis.