Multivariate regression analyses revealed a significant correlation between GC and RNF180 methylation (OR, 3.528; 95% CI, 0.542–0.861; P=0.007) and SFRP2 methylation (OR, 2.647; 95% CI, 1.080–6.487; P=0.033), but not for DAPK1 methylation (OR, 1.540; 95% CI, 0.610–3.890; P=0.361), in GC patients and controls (Table III). The gene discussed is RNF180; the disease is gastric cancer.