The activity of amygdala, a brain structure involved in fear expression and anxiety (Beck and Fibiger, 1995; Ehrlich et al., 2009; Knapska and Maren, 2009; Poulos et al., 2009; Ciocchi et al., 2010; Haubensak et al., 2010; Tye et al., 2011; Mahan and Ressler, 2012; Pare and Duvarci, 2012), was also investigated after PT treatment by immunohistochemical analyses of the expression of the early gene c-fos. This evidence concerns the gene FOS and Anxiety.