LIN28B and Familial prostate cancer: Because miR-212 was the most altered microRNA, it has never been linked functionally to Lin28B in any cancer, and miR-212 was found suppressed in prostate cancer tissues (13), we validated the upregulation of miR-212 by real-time PCR at 24, 48 and 72 h after the Lin28B silencing and the maximum upregulation of miR-212 was detected at 24 h (fold difference, 440.93).