In order to reveal the mechanisms regulating expression in PCa, we have predicted PCGEM1 interaction with miR-145 using billogical information (Figure 1C), and futher investigated a possible interaction with the tumor suppressor miR-145. Co-transfection of LNCaP cells with miR-145 mimics or miR-145 inhibitor with psiCHECK-2-PCGEM1 significantly inhibited reporter gene activity but only miR-145 suppressed reported gene expression when transfected with empty psiCHECK-2 (Figure 1A, B). The gene discussed is PCGEM1; the disease is posterior cortical atrophy.