To examined the effect of siRNA-mediated PCGEM1 knockdown and concomitant overexpression of miR-145 on PCa cell proliferation, normal prostate cells (RWPE–1) and LNCaP cells were transfected as above and cell numbers estimated by MTT after 24, 48, 72 h. The gene discussed is PCGEM1; the disease is posterior cortical atrophy.