In this cross-sectional study we recruited a total of 67 subjects including 27 patients infected with HIV-1 only (age range: 21–53 years and CD4+ T-cell count range: 32–854 cells/μl), 12 patients with active PTB (age range: 21–65 years and CD4+ T-cell count range: 326–1368 cells/μl), 8 HIV patients having pulmonary tuberculosis also (HIV-PTB co-infected patients) (age range: 17–34 years and CD4+ T-cell count range: 30–273 cells/μl), and 20 healthy controls (age range: 22–40 years and CD4+ T-cell count range: 444–1375 cells/μl). This evidence concerns the gene CD4 and pulmonary tuberculosis.