DSG3 and Autoimmunity: We show that, in addition to visually tracking the effects of PV-associated anti-Dsg3 autoantibody binding on cellular adhesion in high resolution and real-time, AFM can reliably detect nanostuctural changes at the intact cell surface in response to pathogenic (blister forming) and non-pathogenic (non blister forming) antibody binding and downstream signaling events that allow conclusions regarding disease pathomechanisms relevant to autoimmunity.