A number of mRNAs were reported as direct targets of miR-1, such as transgelin 2 (TAGLN2) [16,32], purine nucleoside phosphorylase (PNP) [14,32], coding for the cyclin D2 (CCND2), CXC chemokine receptor 4 (CXCR4), stromal cell derived factor-1 (SDF-1) [15], endothelin-1 [12] and fibronectin1 [37] et al. In the research, we validated the targeting of LASP1 (a tumor metastasis-associated protein in CRC), showing that miR-1 may suppress tumors by binding directly in the LASP1 3’UTR. This evidence concerns the gene CXCR4 and colorectal carcinoma.