TP53 and colorectal cancer: To investigate whether miR-339-5p reduces the p53 protein accumulation and p53 transcriptional activity in response to stress in colorectal cancer cells, HCT116 p53+/+ and HCT116 p53−/− cells transfected with miR-339-5p mimic were treated with chemotherapeutic agent 5-Fluorouracil (5-FU), which acted as stress signal to activate p53.