Using this washout protocol, aliquots of CWR-22Rv1, LAPC-4, or LNCaP human prostate cancer cells, as well as human umbilical endothelial cells (i.e., HUVECs) were incubated from 5 minutes to 48 hours with 1 μM 14C-labeled tasquinimod in 10% FBS media containing 0.2 μM free non-albumin bound drug and the PMt determined. Here, ALB is linked to Familial prostate cancer.