To test whether the expression of LGR5 is also associated with Wnt/β-catenin signaling in vivo, the expression of the β-catenin, cyclinD1, and c-myc proteins was examined by a western blot assay in the xenograft tumor tissues formed by LGR5-down-regulated and -overexpressing cervical cancer cells. This evidence concerns the gene CCND1 and cervical carcinoma.