Interestingly, EZH2 inhibition in the TKO cells activated a number of genes identified to be upregulated in t(4;14)+ myeloma patients (Myeloma_MS_subgroup_up) suggesting that loss of H3K27 methylation, through MMSET overexpression or EZH2i, can activate similar gene sets (Supplemental Figure S6C; Supplemental Table S5). This evidence concerns the gene EZH2 and plasma cell myeloma.