The accumulation of CD11b+Ly-6Chigh leukocytes in the brain was slightly, but not significantly, higher in TLR3−/− mice following IC infection of JEV, as compared to wild-type mice, and TLR4−/− mice showed no significant change in leukocyte accumulation by IC infection of JEV (Figure 5H). The gene discussed is TLR4; the disease is infection.