TLR3 and infection: As expected, all TLR3−/− (H-2b) mice succumbed to JE after infection with two different doses of JEV (1.4×107 and 2.8×107 pfu), while wild-type (H-2b) mice showed similar 50% and 70% mortality to wild-type mice of mouse strain (H-2d) used for TLR3−/− mice, respectively (Figure S2A and B).