Further, Tfap2b was also affected by the high-fat diet with up-regulated relative expression levels at 4.78 (SD ±0.67, P>0.01), while Kctd15 was unaffected by obesity (0.99±0.09 (±SD) (Figure 8B). Here, KCTD15 is linked to obesity due to melanocortin 4 receptor deficiency.