GSK3B and brain ischemia: To investigate the influence of the Akt/GSK-3β pathway on the HI brain tissue of the neonatal rats, immunohistochemistry and western blotting were used in the present study to detect p-Akt and GSK-3β expression in the brain tissues 24 h following cerebral ischemia, ELISA was used to determine the brain tissue GSK-3β content, and flow cytometry to determine the rate of neuronal apoptosis.