These results suggested that CS-6 might also partially inhibit the activation of the COX-2 signaling, thereby affecting cell viability.To see whether there was a correlation between the COX-2 inhibition and apoptosis induction mediated by CS-6 in NSCLC cells, we co-treated A549 cells with CS-6 in combination with a COX-2 inhibitor (CB) or a COX-2 inducer (PMA), and then analyzed the expression of COX-2 and the apoptosis-related protein (cleaved caspase-3) by Western blot. The gene discussed is CASP3; the disease is non-small cell lung carcinoma.