IGHE and allergic disease: Although Sanger sequencing has been applied to study IgE repertoires in patients with allergic disease,4, 5 its low sequence yield and limited coverage has resulted in conflicted findings, such as the role of classical T cell–dependent antigens versus superantigens in shaping the selected IgE repertoire in patients with allergic disease.6, 7, 8, 9, 10, 11, 12