None of the other genes tested, TNFα, CXCL-1, F4/80, MRC-1, IL-10, ARG showed changes in the HF mice relative to the LF mice after 3 days or 1 week on diet but all genes tested showed an increase in expression after 16 weeks on a HF diet (Fig 12C–I). Here, TNF is linked to hydrops fetalis.