Five CCA cell lines established from different histological types of primary tumors, namely, KKU-M139, KKU-M156, KKU-M213, KKU-M214 and KKU-100 showed positive expression of p50, p52 and p65 NF-κB subunits (Fig. 1C). The gene discussed is NFKB1; the disease is cholangiocarcinoma.