In this study, we detected phosphorylated forms of the three key constituent proteins on the Akt/mTOR signaling pathway (p-Akt, p-p70S6K and p-4E-BP1) by immunohistochemistry (IHC) in 248 cases of NPC and 66 cases of non-cancerous nasopharyngeal control tissue, and investigated the correlations between the expression of p-Akt, p-p70S6K and p-4EBP1 proteins and clinicopathological features and prognostic implications in NPC. This evidence concerns the gene RPS6KB1 and nasopharyngeal carcinoma.