Surprisingly, this reciprocal trend between ASS1 expression and cisplatin resistance that we observe in our HCC cell lines is not seen with other platinum therapies such as oxaliplatin and carboplatin, or other chemotherapies such as doxorubicin, docetaxel, 5-FU or gemcitabine (data not shown). Here, ASS1 is linked to hepatocellular carcinoma.