In NSCLC, common methodologies used to detect the EGFR mutations are: direct sequencing, PCR-SSCP, mutant-enriched PCR, ARMS, microfluidics digital PCR, HRM, DHPLC, and etc. There are pros and cons in each method; not to mention the rate of mutation is different in each method as well [40]. The gene discussed is EGFR; the disease is non-small cell lung carcinoma.