These significant variables were further subjected to multivariate Cox proportional hazards model, which shown that tumor size(P = 0.008), tumor number(P = 0.032), microvascular invasion(P = 0.019), p-Ser9-GSK-3β expression level(P<0.001) were independent risk factors that could affect the survival of patients with HCC(table 2). This evidence concerns the gene GSK3B and hepatocellular carcinoma.