We firstly assayed and compared GSK-3β mRNA levels in 60 TTs(tumor tissues) and their matched NTTs(nontumorous tissues) by the way of RT-PCR assay (Fig. 1A, B), in which 50 of 60 (83.3%)specimens showed GSK-3β mRNA over-expression in HCC(tumor/nontumorous ratio>2.0). The gene discussed is GSK3B; the disease is neoplasm.