Univariate analysis of 23 survival-related clinic-pathological variables revealed that serum AFP level(P  = 0.024), tumor size(P = 0.003), tumor number(P = 0.022), liver cirrhosis(P = 0.038), microvascular invasion(P = 0.011), p-Ser9-GSK-3β expression level(P<0.001), and T2DM(P = 0.006) were statistically correlated with OS of patients with HCC (Table S1). This evidence concerns the gene GSK3B and hepatocellular carcinoma.