AR and adenocarcinoma: Dual immunohistochemistry for AR and CHGA on the same patient’s diagnostic biopsy (prior to NHT; diagnosis of adenocarcinoma, Gleason 4 + 5) revealed that biopsy contained few CHGA positive cells, while the prostatectomy sample (after 8 months of goserelin and flutamide) harbored few AR positive foci (Figure 1H, Additional file 2: Figure S2).