In contrast to the HFD-induced beneficial effect to the bone quality in AD mice, a higher level of leptin induced by HFD in adult C57BL/6 mice did not lead to enhanced bone mechanical properties (Ionova-Martin et al., 2011), which suggests that leptin acts differently in osteoporosis-involved AD mice than it does in normal controls fed with HFD. This evidence concerns the gene LEP and osteoporosis.