Then, 5 potential target genes with tumor-promoting function (AMFR, NOTCH1, HNRNPF, TOP1, and LAPTM4B) were selected from the 31 genes and their 3′UTRs containing the complementary binding sites of miR-139-5p were cloned into a luciferase reporter vector to evaluate the influence of miR-139-5p on the expression of a reporter gene using a luciferase assay (Fig. 3A). The gene discussed is TOP1; the disease is neoplasm.