ANGPTL4 and hepatocellular carcinoma: To obtain an optimal cutoff point for examining the prognostic value of ANGPTL4 mRNA in HCC patients, Receiver operating characteristic (ROC) curve was generated to analyze the sensitivity and 1-specificity using the value of ΔΔΔCt(ANGPTL4patient) to predict the 1st year postoperative overall survival of HCC patients (Figure 2A).